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Area And Schools. County Or Parish. High School District. The proteins were purified by immobilized metal affinity chromatography, cleavage of the tag, and gel filtration. The structures were solved by molecular replacement with a P.
DNA binding was measured using fluorescence polarization. Using the oligonucleotides listed in Table S1 , kinetic constants were calculated from binding curves with a four-parameter logarithmic binding equation using the program PRISM GraphPad.
Abraham Cephalosporin Fund and Linacre College for support. Sponsored Document from. Structure London, England Joseph A. Newman , 1, 2 Christopher D. Christopher D. Author information Article notes Copyright and License information Disclaimer. Opher Gileadi: ku. Document S2. Article plus Supplemental Information.
Graphical Abstract. Open in a separate window. Table 1 Data Collection and Refinement Statistics. Figure 1. Figure 2. Figure 3. Figure 4. Figure 5. Figure 6. Figure 7. Author Contributions J. Notes Published: December 1, Supplemental Information Document S1. Article plus Supplemental Information: Click here to view. References Arana M. Nucleic Acids Res. Bernstein D.
High-resolution structure of the E. EMBO J. Braithwaite D. Compilation, alignment, and phylogenetic relationships of DNA polymerases. Buttner K. Structural basis for DNA duplex separation by a superfamily-2 helicase. Cannavo E. Ceccaldi R. Homologous-recombination-deficient tumours are dependent on Poltheta-mediated repair. Doherty A. Feldkamp M. Goff J. Lack of DNA polymerase theta POLQ radiosensitizes bone marrow stromal cells in vitro and increases reticulocyte micronuclei after total-body irradiation.
Guy C. Archaeal Hel helicase targets replication forks in vivo and in vitro and unwinds lagging strands. Gyimesi M. Processive translocation mechanism of the human Bloom’s syndrome helicase along single-stranded DNA. Harding S. Royal Society of Chemistry; Analytical Ultracentrifugation in Biochemistry and Polymer Science. Higgins G. Overexpression of POLQ confers a poor prognosis in early breast cancer patients. Cancer Res.
Hogg M. Inagaki S. Plant Cell. Kawamura K. Luke and Martin together with Clarkson face to face. Lyrically, the song narrates the story of a failed relationship which initially started off well. Clarkson regrets having allowed herself to be vulnerable to her ex-boyfriend and she is determined that despite the pain that she feels, he will not get the satisfaction of seeing her cry. Elizabeth Scott of Sky Living wrote, “while Clarkson is doing well musically, her love life still hasn’t picked up and she is heartbroken once again.
I’m sure the thought of another top ten hit might cheer her up! Clarkson’s down-home everything well, mostly her looks sells these little heartbreak haikus exceptionally well. It became the sixteenth ranked single of the s decade on the Pop Songs chart compiled by Billboard. Internationally, “Behind These Hazel Eyes” was a commercial success. In Australia, the song debuted and peaked at number six on the week ending July 3, During Clarkson’s wardrobe fitting, they communicated together using notes written by her.
The concept of the music video was conceived by Clarkson. She explained, “The whole thing is a metaphor about a broken fairy tale [ It’s kind of a sad video, but it’s going to be my best one.
It’s real, and that’s why people like me. The music video begins with Clarkson standing in a room wearing a white wedding dress. Holding a bouquet of red roses, she is surrounded by her bridesmaids as they prepare for her wedding. The bridesmaids are unable to close the windows so they run out of the room leaving Clarkson alone, who is seen dropping the bouquet of rose as well as the wedding photo from her hands onto the floor.
The next scene shows Clarkson walking down the aisle in a church wearing a black dress. When she draws the veil of the bride, she realizes that the bride is the woman from the photo.
Clarkson, who is now wearing a white wedding dress, runs out of the church and into a dark swamp. A montage of Clarkson singing with her band in a dark swamp is shown alternately with scenes of Clarkson running in the forest and lying on the ground.
Clarkson is also seen standing inside an abandoned house, wearing a voluminous black dress. As the rain starts to fall, Clarkson finally collapses, kneeling on the ground while looking at and being comforted by her other self who is singing with the band. Before he puts the ring on her finger, she looks at the guests and notices that the woman from the photo is among the audience. She throws her wedding bouquet into the lap of the woman and shoves her way past the wedding guests who are trying to stop her from leaving.
The video ends with Clarkson stepping out the doors into the bright sunlight. James Dinh of MTV Newsroom listed “Behind These Hazel Eyes” as one of the music videos with “a bad case of the runaway bride syndrome,” writing, “Throughout the clip, Clarkson finds herself running through a swampy mud land until she snaps out of her visions, refuses to proceed with the wedding and makes a shocking escape out of the church.
Music put the song’s music video at number 22 on her list of the best matrimony-themed musical videos of all time. He said, “She is so unbelievably gorgeous in that video that is all over TV right now [ Who knew? She is so hot. She looks so good in it.
During her concert tour at Germain Arena , Clarkson performed the song wearing the same wedding dress she wore in the song’s music video. While touring at Hammerstein Ballroom , New York City, Clarkson sang the stripped down version of the song, accompanied by guitars and vocal.
Bob Gendron of the Chicago Tribune reviewed Clarkson’s performance, writing “the Texas native could’ve impressed simply by projecting her booming voice and relishing its prodigious range. Yet she seemed determined to reinforce female empowerment themes often missing from her mainstream-pop contemporaries’ hits. According to Pope, she decided to sing the song in order to reach out to her father who divorced from her mother when she was still 11 years old.
Credits adapted from the liner notes of Breakaway. From Wikipedia, the free encyclopedia. Not to be confused with Behind Hazel Eyes. Maratone Stockholm Westlake Los Angeles. Max Martin Dr. The second chorus of “Behind These Hazel Eyes” which features the dominant sound of electric guitar.
Problems playing this file? See media help. Kelly Clarkson — lead vocals and background vocals Shawn Pelton – drums, background vocals Dr. The New York Times Company. Archived from the original on May 12, Retrieved March 6, Prometheus Global Media. Entertainment Weekly. Time Inc. Retrieved March 7, Pop Crush.
Retrieved February 29, May 1, Archived from the original on December 24, Retrieved April 30, Alfred Publishing. February 7, Technorati, Inc. Retrieved January 16, CBS Interactive. Burlee LLC. Archived from the original on December 13, Guardian Media Group. British Sky Broadcasting. Sarah Zupko. Stylus Magazine. Todd Burns. Cox Communications. Cox Enterprises.
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